Pcr Protocols: A Guide to Methods and Applications

The broad utility of the polymerase chain reaction (PCR) method is now within the reach of every researcher. Designed for use at the laboratory bench, this is the most comprehensive manual on PCR available today. Over 50 chapters provide precise instructions on procedures, with advice on primer design. All of the techniques described, from amplification and direct sequencing of genomic DNA through cDNA cloning and quantitation of mRNA, are tested, current, and supplemented with helpful notes and illustrations. You'll also learn how to: optimize novel applications; avoid many cumbersome molecular biological techniques; and set up the laboratory to avoid contamination. This first-rate guide will help you: avoid contamination - with specific instructions on setting up your lab; avoid cumbersome molecular biological techniques; and, discover new applications.